ISCB-Asia/SCCG 2012, session on cancer genome informatics


Biaoyang Lin
Zhejiang-California International Nanosystems Institute (ZCNI), Zhejiang University

Recurrent targeted genes of hepatitis B virus in the liver cancer genomes identified by a next-generation sequencing based approach

Abstract

Integration of the viral DNA into host chromosomes was found in most of the hepatitis B virus (HBV) related hepatocellular carcinomas (HCCs). Here we devised a massive anchored parallel sequencing (MAPS) method using next-generation sequencing to isolate and sequence HBV integrants. Applying MAPS to HBV-related HCC tissues (cancer and adjacent tissues), we identified 296 HBV integration events corresponding to 286 unique integration sites (UISs) with precise HBV-Human DNA junctions. We identified 11 genes that were recurrent target genes by HBV integration including fibronectin 1 (FN1) and telomerase reverse transcriptase (TERT1), two known recurrent target genes, and additional novel target genes. This global survey of HBV integration events, together with recently published whole genome sequencing analyses, furthered our understanding of the HBV-related HCC.

Biography

Prof. Biaoyang Lin is the Director of Systems Biology at Zhejiang-California International Nanosystems Institute (ZCNI), Zhejiang University, Hangzhou, China.

He recieved his Ph.D. in Medical Genetics from the University of British Columbia, Canada, 1995 and a Humboldt fellowship in Germany, 1996. He has been awarded six US patents. He is Associate Editor of OMICS: A Journal of Integrative Biology; Editor-in-Chief, Recent Patents on Biomedical Engineering.

His research interests include systems biology, bioinformatics, genomics and proteomics. He has published over 60 papers in PNAS, Science, Nature, New Eng. J. Medicine, Cancer Research, molecular and cellular proteomics etc. They were cited over 3750 times including seven that were cited over 100 times.